Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns
نویسندگان
چکیده
■ ■ 2.5 μm particle size provides the flexibility to operate on a variety of systems IN T RO DU C T IO N Hydrophilic-interaction chromatography (HILIC) is increasingly becoming a method of choice for the analysis of polar compounds [1-5]. One set of polar analytes that poses particular challenges are the monoamine neurotransmitters, dopamine (DA), serotonin (5-HT), epinephrine (EP), and norepinephrine (NE). These compounds play a significant role in many mood, movement, and other neurological disorders such as depression, anxiety, schizophrenia, and Parkinson’s disease[6-8]. These neurotransmitters also play a critical role in the effects and toxicity of drugs of abuse[9-11].
منابع مشابه
Hydrophilic interaction chromatography (HILIC) for LC-MS/MS analysis of monoamine neurotransmitters.
BACKGROUND Hydrophilic interaction chromatography (HILIC) is becoming an increasingly popular alternative to traditional reversed-phase chromatography for the analysis of polar compounds. The ability to retain the most polar compounds in HILIC makes it attractive for the analysis of certain large groups of compounds, such as monoamines, which are inherently very polar. RESULTS This paper deta...
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